mycbp  (Proteintech)

Journal: bioRxiv

Article Title: Proteomic composition and mutual assembly of the C2a projection in vertebrate motile cilia

doi: 10.64898/2026.01.24.701544

Figure Lengend Snippet: ( A , B ) Silver staining of proteins immunoprecipitated from mouse testis lysates using a normal guinea pig IgG and a guinea pig polyclonal anti-MYCBPAP antibody ( A ) or a normal rat IgG control antibody and a rat polyclonal anti-CFAP70 antibody ( B ). The bands of MYCBPAP and CFAP70 are indicated by red arrowheads. ( C , D ) Interactor candidates of MYCBPAP ( C ) and CFAP70 ( D ) identified by mass spectrometry analysis. ( E , F ) Co-IP and immunoblotting showing the interactions of endogenous MYCBPAP ( E ) and CFAP70 ( F ) with ARMC3 and MYCBP. Co-IP was performed with a normal guinea pig IgG and a guinea pig polyclonal anti-MYCBPAP antibody ( E ) or a normal rat IgG control antibody and a rat polyclonal anti-CFAP70 antibody ( F ) in mouse testis lysates. ( G , H ) Co-IP and immunoblotting analyses in HEK293T cells exogenously expressing indicated proteins. GFP-tagged proteins were immunoprecipitated with anti-GFP agarose beads. Blots were probed with the indicated antibodies. Luci, luciferase. ( I ) 3D-SIM images of mEPCs immunostained with the indicated antibodies. Magnified images of the cilia indicated by arrowheads are shown on the right. Line-scan graphs show the immunofluorescence intensity along the positions marked by two arrows in the magnified images. ( J – M ) Representative confocal images of mEPC from WT, Mycbpap , and Cfap70 KO mice immunostained with the indicated antibodies.

Article Snippet: The following primary antibodies were used: acetylated α-tubulin (T6793, Sigma-Aldrich; IF 1:500, WB 1:5000), GAPDH (10494-1-AP, Proteintech; WB 1:20000), GFP (this study; IF 1:200, WB 1:5000), HA (M20003M, Abmart; WB 1:10000), ARMC3 (28418-1-AP, Proteintech; IF 1:200, WB 1:200), MYCBP (12022-1-AP, Proteintech; IF 1:200, WB 1:200), CEP164 (22227-1-AP, Proteintech; IF 1:500), CEP164 (17603-1-AP, Proteintech; IF 1:200), His (HRP-66005, Proteintech; WB 1:5000), CEP164 (this study; IF 1:1000), MYCBPAP (this study; IF 1:500, WB 1:1000), CFAP70 (this study; IF 1:500, WB 1:1000), CCDC108 (this study; IF 1:500), SPEF1 (this study; IF 1:1000), HYDIN (this study; IF 1:200), ODF2 (this study; IF 1:1000).

Techniques: Silver Staining, Immunoprecipitation, Control, Mass Spectrometry, Co-Immunoprecipitation Assay, Western Blot, Expressing, Luciferase, Immunofluorescence

Journal: bioRxiv

Article Title: Proteomic composition and mutual assembly of the C2a projection in vertebrate motile cilia

doi: 10.64898/2026.01.24.701544

Figure Lengend Snippet: ( A , B ) Silver staining of proteins immunoprecipitated from mouse testis lysates using a normal guinea pig IgG and a guinea pig polyclonal anti-MYCBPAP antibody ( A ) or a normal rat IgG control antibody and a rat polyclonal anti-CFAP70 antibody ( B ). The bands of MYCBPAP and CFAP70 are indicated by red arrowheads. ( C , D ) Interactor candidates of MYCBPAP ( C ) and CFAP70 ( D ) identified by mass spectrometry analysis. ( E , F ) Co-IP and immunoblotting showing the interactions of endogenous MYCBPAP ( E ) and CFAP70 ( F ) with ARMC3 and MYCBP. Co-IP was performed with a normal guinea pig IgG and a guinea pig polyclonal anti-MYCBPAP antibody ( E ) or a normal rat IgG control antibody and a rat polyclonal anti-CFAP70 antibody ( F ) in mouse testis lysates. ( G , H ) Co-IP and immunoblotting analyses in HEK293T cells exogenously expressing indicated proteins. GFP-tagged proteins were immunoprecipitated with anti-GFP agarose beads. Blots were probed with the indicated antibodies. Luci, luciferase. ( I ) 3D-SIM images of mEPCs immunostained with the indicated antibodies. Magnified images of the cilia indicated by arrowheads are shown on the right. Line-scan graphs show the immunofluorescence intensity along the positions marked by two arrows in the magnified images. ( J – M ) Representative confocal images of mEPC from WT, Mycbpap , and Cfap70 KO mice immunostained with the indicated antibodies.

Article Snippet: The following primary antibodies were used: acetylated α-tubulin (T6793, Sigma-Aldrich; IF 1:500, WB 1:5000), GAPDH (10494-1-AP, Proteintech; WB 1:20000), GFP (this study; IF 1:200, WB 1:5000), HA (M20003M, Abmart; WB 1:10000), ARMC3 (28418-1-AP, Proteintech; IF 1:200, WB 1:200), MYCBP (12022-1-AP, Proteintech; IF 1:200, WB 1:200), CEP164 (22227-1-AP, Proteintech; IF 1:500), CEP164 (17603-1-AP, Proteintech; IF 1:200), His (HRP-66005, Proteintech; WB 1:5000), CEP164 (this study; IF 1:1000), MYCBPAP (this study; IF 1:500, WB 1:1000), CFAP70 (this study; IF 1:500, WB 1:1000), CCDC108 (this study; IF 1:500), SPEF1 (this study; IF 1:1000), HYDIN (this study; IF 1:200), ODF2 (this study; IF 1:1000).

Techniques: Silver Staining, Immunoprecipitation, Control, Mass Spectrometry, Co-Immunoprecipitation Assay, Western Blot, Expressing, Luciferase, Immunofluorescence